Detection of deoxynivalenol in foods and indoor air using an array biosensor.

Deoxynivalenol (DON), a mycotoxin produced by several Fusaruim species, is a worldwide contaminant of foods and feeds. Because of the potential dangers due to accidental or intentional contamination of foods with DON, there is a need to develop a rapid and highly sensitive method for easy identification and quantification of DON. In this study, we have developed and utilized a competitive immunoassay technique to detect DON in various food matrixes and indoor air samples using an array biosensor. A DON-biotin conjugate, immobilized on a NeutrAvidin-coated optical waveguide, competed with the DON in the sample for binding to fluorescently labeled DON monoclonal antibodies. To demonstrate a simple procedure amenable for on-site use, DON-spiked cornmeal, cornflakes, wheat, barley, and oats were extracted with methanol-water (3:1) and assayed without cleanup or preconcentration. The limits of detection ranged from 0.2 ng/mL in buffer to 50 ng/g in oats. The detection limit of DON spiked into an aqueous effluent from an air sampler was 4 ng/mL.

Ngundi, M. M., Qadri, S. A., Wallace, E. V., Moore, M. H., Lassman, M. E., Shriver-Lake, L. C., Ligler, F. S., & Taitt, C. R.

Ngundi, Miriam M||Qadri, Syed A||Wallace, Elizabeth V||Moore, Martin H||Lassman, Michael E||Shriver-Lake, Lisa C||Ligler, Frances S||Taitt, Chris R

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