Fabrication of key components of a receptor-based biosensor.

abstract

• A three-pronged approach was taken to the development of receptor-based bisensors. First, asymmetric bilayer membranes were developed with one monolayer adaptable to the particular receptor of interest and the other monolayer polymerized to enhance membrane stability. Second, alamethicin and calcium channel complexes were introduced into the stabilized membrane and tested for ion-channel function. Third, a porous support for the receptor-containing membrane was fabricated, which is compatible with silicon technology. Preliminary devices incorporating these components were constructed.

authors

• Ligler, Frances

published proceedings

• Med Instrum

author list (cited authors)

• Ligler, F. S., Fare, T. L., Seib, K. D., Smuda, J. W., Singh, A., Ahl, P., ... Yager, P.

citation count

• 31

complete list of authors

• Ligler, FS||Fare, TL||Seib, KD||Smuda, JW||Singh, A||Ahl, P||Ayers, ME||Dalziel, A||Yager, P

publication date

• October 1988

published in

• Medical instrumentation  Journal

keywords

• Alamethicin
• Animals
• Biosensing Techniques
• Calcium Channels
• Cattle
• Electric Conductivity
• Electrodes
• Equipment Design
• Ligands
• Lipid Bilayers
• Receptors, Cell Surface
• Surface Properties

PubMed Central ID

• 2846991

start page

• 247

end page

• 256

volume

• 22

issue

• 5