Rapid detection of D-dimer using a fiber optic biosensor. Academic Article uri icon

abstract

  • We describe a rapid and sensitive method for detection and quantification of D-dimer and other crosslinked fibrin degradation products (XL-FDPs), which are present in elevated concentrations in patients with sepsis and thrombotic disorders. The method utilizes a sandwich fluoroimmunoassay immobilized in the sensing region of an evanescent wave biosensor. Physiological concentrations of D-dimer and high molecular weight XL-FDP could be determined in buffer and plasma samples on calibrated fibers in 11 min. Samples from septic patients were assayed using ELISA and the fiber optic method; concentrations determined by fiber optic assay were strongly correlated with those determined by ELISA (r = 0.918); intra- and inter-assay errors were comparable to those from ELISAs. Given its accuracy and rapid response time, this fiber optic biosensor shows great potential for development as a diagnostic tool.

published proceedings

  • Thromb Haemost

altmetric score

  • 3

author list (cited authors)

  • Rowe, C. A., Bolitho, J. S., Jane, A., Bundesen, P. G., Rylatt, D. B., Eisenberg, P. R., & Ligler, F. S.

citation count

  • 25

complete list of authors

  • Rowe, CA||Bolitho, JS||Jane, A||Bundesen, PG||Rylatt, DB||Eisenberg, PR||Ligler, FS

publication date

  • January 1998

publisher