A Micro-Flow Cytometer Based on Dielectrophoretic Particle Focusing Conference Paper uri icon

abstract

  • This paper reports an on-chip micro- flow cytometer that focuses cells to the center region of a microchannel by a negative dielectrophoretic force generated by ac fringing fields from microelectrodes. This design eliminates the sheath flow that makes the conventional cytometer sophisticated and difficult to operate. The micro- cytometer has a circular microfluidic channel that is fabricated by isotropic etching of soda lime glass wafers and a subsequent wafer bonding process. Electrode arrays are patterned inside the circular channel to facilitate dielectrophoretic particle focusing. We have demonstrated that the device can focus micro-beads into the center of the channel, and are integrating optical components with the micro- cytometer to realize a handheld device with integrated fluorescence detection, and will test the micro- cytometer for blood cell profiling and tumor cell detection.

name of conference

  • ASME 2003 International Mechanical Engineering Congress and Exposition

published proceedings

  • Microelectromechanical Systems

author list (cited authors)

  • Yu, C., Shi, L. i., Vykoukal, J., & Gascoyne, P.

citation count

  • 0

complete list of authors

  • Yu, Choongho||Shi, Li||Vykoukal, Jody||Gascoyne, Peter RC

publication date

  • January 2003

publisher