Scanning ion conductance microscopy measurement of paracellular channel conductance in tight junctions.

abstract

• Elucidation of epithelial transport across transcellular or paracellular pathways promises to advance the present understanding of ion transport and enables regulation of cell junctions critical to the cell and molecular biology of the epithelium. Here, we demonstrate a new instrumental technique, potentiometric scanning ion conductance microscopy (P-SICM), that utilizes a nanoscale pipet to differentiate paracellular and transcellular transport processes at high spatial resolution. The technique is validated for well-defined polymer membranes and then employed to study wild type and claudin-deficient mutants of Madin-Darby Canine Kidney strain II (MDCKII) cells. Paracellular permeabilities conferred by claudin-2 are captured by P-SICM which demonstrates the utility to monitor apparent conductance at subcellular levels.

authors

• Baker, Lane

published proceedings

• Anal Chem

altmetric score

• 0.5

author list (cited authors)

• Chen, C., Zhou, Y. i., Morris, C. A., Hou, J., & Baker, L. A.

citation count

• 59

complete list of authors

• Chen, Chiao-Chen||Zhou, Yi||Morris, Celeste A||Hou, Jianghui||Baker, Lane A

publication date

• April 2013

publisher

• American Chemical Society (ACS)  Publisher

published in

• Analytical Chemistry  Journal

keywords

• Animals
• Cell Line
• Claudin-2
• Dogs
• Electrochemical Techniques
• Epithelium
• Equipment Design
• Ion Transport
• Ions
• Microscopy
• Permeability
• Tight Junctions

PubMed Central ID

• 23421780

Digital Object Identifier (DOI)

• 10.1021/ac303441n

start page

• 3621

end page

• 3628

volume

• 85

issue

• 7

URL

• http://dx.doi.org/10.1021/ac303441n