Ion channel probes for scanning ion conductance microscopy. Academic Article

abstract

• The sensitivity and selectivity of ion channels provide an appealing opportunity for sensor development. Here, we describe ion channel probes (ICPs), which consist of multiple ion channels reconstituted into lipid bilayers suspended across the opening of perflourinated glass micropipets. When incorporated with a scanning ion conductance microscope (SICM), ICPs displayed a distance-dependent current response that depended on the number of ion channels in the membrane. With distance-dependent current as feedback, probes were translated laterally, to demonstrate the possibility of imaging with ICPs. The ICP platform yields several potential advantages for SICM that will enable exciting opportunities for incorporation of chemical information into imaging and for high-resolution imaging.

authors

• Baker, Lane

published proceedings

• Langmuir

altmetric score

• 3

author list (cited authors)

• Zhou, Y. i., Bright, L. K., Shi, W., Aspinwall, C. A., & Baker, L. A.

citation count

• 25

complete list of authors

• Zhou, Yi||Bright, Leonard K||Shi, Wenqing||Aspinwall, Craig A||Baker, Lane A

publication date

• December 2014

publisher

• American Chemical Society (ACS)  Publisher

published in

• Langmuir  Journal

keywords

• Biosensing Techniques
• Electric Conductivity
• Feedback
• Ion Channels
• Lipid Bilayers
• Microscopy, Scanning Probe
• Models, Molecular
• Protein Conformation

PubMed Central ID

• 25425190

Digital Object Identifier (DOI)

• 10.1021/la504097f

start page

• 15351

end page

• 15355

volume

• 30

issue

• 50

URL

• http://dx.doi.org/10.1021/la504097f