Ion concentration in micro and nanoscale electrospray emitters.

abstract

• Solution-phase ion transport during electrospray has been characterized for nanopipettes, or glass capillaries pulled to nanoscale tip dimensions, and micron-sized electrospray ionization emitters. Direct visualization of charged fluorophores during the electrospray process is used to evaluate impacts of emitter size, ionic strength, analyte size, and pressure-driven flow on heterogeneous ion transport during electrospray. Mass spectrometric measurements of positively- and negatively-charged proteins were taken for micron-sized and nanopipette emitters under low ionic strength conditions to further illustrate a discrepancy in solution-driven transport of charged analytes. A fundamental understanding of analyte electromigration during electrospray, which is not always considered, is expected to provide control over selective analyte depletion and enrichment, and can be harnessed for sample cleanup. Graphical abstract Fluorescence micrographs of ion migration in nanoscale pipettes while solution is electrosprayed.

authors

• Baker, Lane

published proceedings

• Anal Bioanal Chem

author list (cited authors)

• Yuill, E. M., & Baker, L. A.

complete list of authors

• Yuill, Elizabeth M||Baker, Lane A

publication date

• June 2018

publisher

• Springer Nature  Publisher

published in

• Analytical and Bioanalytical Chemistry  Journal

keywords

• Electroanalytical Methods
• FLUORESCENCE
• Ion Transport
• Ions
• Microfluidic Analytical Techniques
• Microfluidics / Microfabrication
• Motion
• Nanoparticles / Nanotechnology
• Osmolar Concentration
• Pressure
• Proteins
• Spectrometry, Mass, Electrospray Ionization

PubMed Central ID

• 29704029

Digital Object Identifier (DOI)

• 10.1007/s00216-018-1043-5

start page

• 3639

end page

• 3648

volume

• 410

issue

• 16

URL

• http://dx.doi.org/10.1007/s00216-018-1043-5