This paper experimentally studies the effects of the buoyancy force and channel aspect ratio on heat transfer in two-pass rotating rectangular channels with smooth walls and 45° ribbed walls. The channel aspect ratios include 4:1, 2:1, 1:1, 1:2 and 1:4. Four Reynolds numbers are studied: 5000, 10000, 25000 and 40000. The rotation speed is fixed at 550 rpm for all tests, and for each channel, two channel orientations are studied: 90° and 45° or 135°, with respect to the plane of rotation. Rib turbulators are placed on the leading and trailing walls of the channels at an angle of 45° to the flow direction. The ribs have a 1.59 by 1.59 mm square cross section, and the rib pitch-to-height ratio (P/e) is 10 for all tests. The effects of the local buoyancy parameter and channel aspect ratio on the regional Nusselt number ratio are presented. The results show that increasing the local buoyancy parameter increases the Nusselt number ratio on the trailing surface and decreases the Nusselt number ratio on the leading surface in the first pass for all channels. However, the trend of the Nusselt number ratio in the second pass is more complicated due to the strong effect of the 180° turn. Results are also presented for this critical turn region of the two-pass channels. In addition to these regions, the channel averaged heat transfer, friction factor, and thermal performance are determined for each channel. With the channels having comparable Nusselt number ratios, the 1:4 channel has the superior thermal performance because it incurs the least pressure penalty.