The role of prostaglandins in the development of refractoriness to LH stimulation by Graafian follicles. Academic Article

abstract

• The possibility that prostaglandins might be responsible for the development of the pre-ovulatory refractoriness to the stimulation by LH of cyclic AMP accumulation in vitro in the Graafian follicle was examined. Isolated rabbit Graafian follicles were obtained at estrus and at 0.5, 5 and 9 hours after an ovulatory dose of LH. The follicles were incubated in vitro in the presence of (8-3H)adenine and the accumulation of (8-3H)cyclic AMP measured. Follicles from estrous animals responded to the in vitro addition of LH with a marked increase of cyclic AMP accumulation and lost this response as the time of ovulation approached. Animals pretreated with indomethacin, which inhibits the usual pre-ovulatory rise of follicular prostaglandin levels, showed essentially the same loss of responsiveness. Indomethacin alone was without effect. It is concluded that prostaglandins are not the major factor in the development of refractoriness to LH stimulation in vitro which has been observed in pre-ovulatory follicles.

authors

• Davies, Peter

published proceedings

• Prostaglandins

author list (cited authors)

• LeMaire, W. J., Davies, P. J., & Marsh, J. M.

citation count

• 6

complete list of authors

• LeMaire, WJ||Davies, PJ||Marsh, JM

publication date

• January 1976

publisher

• Elsevier  Publisher

published in

• Prostaglandins  Journal

keywords

• Animals
• Cyclic AMP
• Female
• Indomethacin
• Luteinizing Hormone
• Ovarian Follicle
• Ovulation
• Prostaglandins F
• Rabbits
• Stimulation, Chemical
• Time Factors

PubMed Central ID

• 183241

Digital Object Identifier (DOI)

• 10.1016/0090-6980(76)90121-0

start page

• 271

end page

• 279

volume

• 12

issue

• 2

URL

• http://dx.doi.org/10.1016/0090-6980(76)90121-0