Chloroplast genetic engineering to improve agronomic traits. Chapter

abstract

• Major crop losses occur annually as a result of biotic and abiotic stresses. The ability to hyperexpress foreign proteins, single-step multigene engineering, lack of positive effect and gene silencing, vector sequences and pleiotropic effects have resulted in several hundred-fold more tolerance to the environmental stresses via chloroplast genetic engineering than nuclear genetic engineering. Maternal inheritance of chloroplast expressed transgenes renders the technology environmentally safe and promotes public acceptance. This review provides protocols for engineering agronomic traits like insect, herbicide and disease resistance; salt and drought tolerance; and phyto-remediation via chloroplast genome.

authors

• Dhingra, Amit

author list (cited authors)

• Daniell, H., Ruiz, O. N., & Dhingra, A.

citation count

• 82

complete list of authors

• Daniell, Henry||Ruiz, Oscar N||Dhingra, Amit

Book Title

• Transgenic Plants

publication date

• January 2005

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Animals
• Biolistics
• Blotting, Southern
• Chloroplasts
• Crops, Agricultural
• Genetic Engineering
• INSECTA
• Immunity, Innate
• Plant Diseases
• Plant Leaves
• Plants, Genetically Modified
• Recombination, Genetic
• Tetradecanoylphorbol Acetate
• Tobacco

PubMed Central ID

• 15310917

Digital Object Identifier (DOI)

• 10.1385/1-59259-827-7:111

start page

• 111

end page

• 138

volume

• 286

URL

• http://dx.doi.org/10.1385/1-59259-827-7:111