Multigene engineering: dawn of an exciting new era in biotechnology. Academic Article

abstract

• Development of a rice variety enriched in provitamin A, the accumulation of polyhydroxybutyrate polyester in Arabidopsis nuclear transgenic plants (with enzymes targeted to chloroplasts in both), and the expression of bacterial operons via the chloroplast genome are recent landmark achievements in multigene engineering. Hyper-expression of transgenes has resulted in the formation of insecticidal protein crystals or inclusion bodies of pharmaceutical proteins in transgenic chloroplasts, achieving the highest level of transgene expression ever reported in transgenic plants. These achievements illustrate the potential of multigene engineering to realize benefits of the post-genomic revolution.

authors

• Dhingra, Amit

published proceedings

• Curr Opin Biotechnol

altmetric score

• 12

author list (cited authors)

• Daniell, H., & Dhingra, A.

citation count

• 105

complete list of authors

• Daniell, Henry||Dhingra, Amit

publication date

• January 2002

publisher

• Elsevier  Publisher

published in

• Current Opinion in Biotechnology  Journal

keywords

• Beta Carotene
• Biopharmaceutics
• Biotechnology
• Chloroplasts
• Gene Silencing
• Genetic Engineering
• Multigene Family
• Oryza
• Plant Development
• Plants
• Plants, Genetically Modified
• Transformation, Genetic
• Transgenes

PubMed Central ID

• 11950565

Digital Object Identifier (DOI)

• 10.1016/s0958-1669(02)00297-5

start page

• 136

end page

• 141

volume

• 13

issue

• 2

URL

• http://dx.doi.org/10.1016/s0958-1669(02)00297-5