Plasmid Sequence and Availability for an Improved Clostridioides difficile CRISPR-Cas9 Mutagenesis System. Academic Article uri icon


  • A two-plasmid mutagenesis system for Clostridioides difficile is described that improves ease of use and efficiency in creating site-directed mutations. pJB06 contains a xylose-inducible cas9 gene, while the second plasmid (pJB07) encodes the corresponding guide RNA (gRNA) and regions of homology for repair of the introduced double-stranded DNA (dsDNA) breaks, both of which are replaceable via restriction digest.

published proceedings

  • Microbiol Resour Announc

altmetric score

  • 0.25

author list (cited authors)

  • Brehm, J. N., & Sorg, J. A.

citation count

  • 1

complete list of authors

  • Brehm, Joshua N||Sorg, Joseph A

editor list (cited editors)

  • Newton, I.

publication date

  • January 2022