Wound swabs versus biopsies to detect methicillin resistant Staphylococcus aureus in experimental equine wounds.
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OBJECTIVE: To compare: (1) the load and diversity of cultivatable bacterial species isolated from tissue biopsies with cultures from surface swabs, and (2) the ability of each technique to detect methicillin-resistant Staphylococcus aureus (MRSA) in a model of MRSA-infected equine wounds. STUDY DESIGN: Experimental in vivo study. ANIMALS: Three light-breed adult horses. METHODS: Four 2.52.5cm full-thickness skin wounds were created on the dorsolateral aspect of each forelimb. Five days later, each wound was inoculated with a pure culture of MRSA (ATCC 43300). One hundred microlitres of 0, 5108 , 5109 or 51010 colony forming units (CFU)/ml was used to inoculate each wound. Surface swabs (Levine technique) and tissue biopsy samples (3mm punch biopsy) were obtained at 2, 7, 14, and 21days after inoculation. Quantitative aerobic culture was performed using routine clinical techniques. RESULTS: A similar bacterial profile was identified from the culture of each wound-sampling technique and there was moderate correlation (R=0.49, P<.001) between the bacterial bioburdens. Agreement was fair (=0.31; 95% CI, 0.129-0.505) between the sampling techniques in identification of MRSA. Methicillin-resistant Staphylococcus aureus was isolated more frequently (P=.016) from cultures of tissue biopsies (79%; 76/96) than from surface swabs (62%; 60/96). CONCLUSION: Bacterial load and diversity did not differ between sampling techniques but MRSA was detected more often from the cultures of tissue biopsies. CLINICAL SIGNIFICANCE: Tissue biopsy should be preferred to culture swab in wounds where MRSA is suspected.
Brock, A. K., Chamoun-Emanuelli, A. M., Howard, E. A., Huntzinger, K. D., Lawhon, S. D., Bryan, L. K., ... Whitfield-Cargile, C. M.
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Brock, Abbi K||Chamoun-Emanuelli, Ana M||Howard, Emily A||Huntzinger, Katie D||Lawhon, Sara D||Bryan, Laura K||Cosgriff-Hernandez, Elizabeth M||Cohen, Noah D||Whitfield-Cargile, Canaan M
