Characterisation of gram negative bacterial isolates from guts of few multivoltine silkworm breeds

The members of gram negative bacteria harbouring the silkworm are considered to be functionally important for improving silk yield and quality. The gut microbiology of lepidopteran insects received less attention in the earlier decades as it was thought that owing to their simple digestive tract, they do not harbour significant microbial load. However, recent studies using tools of biochemistry and molecular biology allow identification of new groups of microbes which have hitherto been unexplored. The present study focuses on the characterisation of gram negative microbes from the gut of silkworm using rapid identification kit, KB002 HiAssorted TM (Himedia Laboratories Pvt. Ltd., Mumbai). The gram negative bacteria identified and characterized from indigenous silkworm breeds using the rapid biochemical test kit were Escherichia coli, Yersinia enterocolitica and Klebsiella, Pneumaniae sp. pneumoniae. The details of isolation and characterization are discussed in this paper.

Characterisation of gram negative bacterial isolates from guts of few multivoltine silkworm breeds Academic Article