Derivation and Characterization of Mesenchymal Stem Cells from iPS Cells. Chapter

abstract

• Mesenchymal stem/stromal cells (MSCs) show excellent therapeutic potentials in many preclinical studies and clinical trials. However, the clinical application of conventional tissue-derived MSCs faces challenges of limited scalability and high donor variations. To address these challenges, we established a protocol for deriving and characterizing MSCs from human induced pluripotent stem cells (iPSCs) with a theoretically limitless expandability. The iPSC-MSCs show biological properties comparable to or better than early passage bone marrow MSCs and can be scaled up to huge amounts with uniform properties.

authors

• Liu, Fei

author list (cited authors)

• Zhao, Q., & Liu, F.

citation count

• 2

complete list of authors

• Zhao, Qingguo||Liu, Fei

Book Title

• Induced Pluripotent Stem (iPS) Cells

publication date

• January 2022

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Cell Differentiation
• Characterization
• Derivation
• Humans
• Induced Pluripotent Stem Cells
• Mesenchymal Stem Cells
• Mesenchymal Stem/stromal Cells
• Scalability

PubMed Central ID

• 33058029

Digital Object Identifier (DOI)

• 10.1007/7651_2020_327

International Standard Book Number (ISBN) 13

• 9781071621189

start page

• 443

end page

• 454

volume

• 2454

URL

• http://dx.doi.org/10.1007/7651_2020_327