Creating new specific ligand-receptor pairs for transgene regulation. Academic Article

abstract

• The creation of specifically matched ligand-receptor pairs that are orthogonal to naturally present interacting pairs is essential for the development of small molecule-regulated gene expression systems for biotechnological applications. However, for many years this task has represented a significant challenge for synthetic chemists and protein engineers. Recently, Doyle and colleagues demonstrated that highly specific ligand-receptor pairs can be engineered in a rapid fashion by creating large libraries of protein variants and applying a selection scheme to identify variants with improved activation by the target synthetic ligand.

authors

• Chockalingam, Karuppiah

published proceedings

• Trends Biotechnol

author list (cited authors)

• Chockalingam, K., & Zhao, H.

citation count

• 11

complete list of authors

• Chockalingam, Karuppiah||Zhao, Huimin

publication date

• July 2005

publisher

• Elsevier  Publisher

published in

• Trends in Biotechnology  Journal

keywords

• Binding Sites
• Biochemistry
• Biotechnology
• Gene Expression Regulation
• Ligands
• Protein Engineering
• Retinoid X Receptors
• Transgenes

PubMed Central ID

• 15978316

Digital Object Identifier (DOI)

• 10.1016/j.tibtech.2005.05.002

start page

• 333

end page

• 335

volume

• 23

issue

• 7

URL

• http://dx.doi.org/10.1016/j.tibtech.2005.05.002