Detecting Three-Dimensional Vascular Networks in the Mouse Embryonic Hindbrain. Chapter

abstract

• Blood vessel formation is a fine-regulated process and interfering with blood vessel formation causes embryonic lethality as well as associated with many diseases in the adult, including inflammatory, ischemic, and cancer metastatic diseases. Brain contains abundant blood vessels and has some unique physiological functions, such as blood-brain barrier. Due to the thickness and opaque characters of the tissues, it is a challenge to visualize the three-dimensional structures of the brain blood vessels in the mouse. Therefore, establishing a protocol to display the three-dimensional structures in the brain is required for exploring the regulatory molecular mechanisms in brain blood vessel formation. In this manuscript, we introduced a whole-mount and a vibratome thick section of mouse embryonic hindbrain to display the three-dimensional structures of brain vascular system.

authors

• Peng, Xu

author list (cited authors)

• Huang, C., Wu, L., Wang, J., Tharakan, B., & Peng, X. u.

citation count

• 0

complete list of authors

• Huang, Chenshen||Wu, Liangjing||Wang, Jian||Tharakan, Binu||Peng, Xu

Book Title

• Cardiovascular Development

publication date

• January 2021

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Angiogenesis
• Animals
• Blood Vessels
• Dissection
• Endothelial Cells
• Fluorescent Antibody Technique
• Hindbrain
• Mice
• Microscopy, Confocal
• Neovascularization, Physiologic
• Rhombencephalon
• Staining And Labeling
• Vascular Endothelial Cells
• Vibratome Section
• Whole-mount Staining

PubMed Central ID

• 34331246

Digital Object Identifier (DOI)

• 10.1007/978-1-0716-1480-8_10

International Standard Book Number (ISBN) 13

• 9781071614792

start page

• 87

end page

• 92

volume

• 2319

URL

• http://dx.doi.org/10.1007/978-1-0716-1480-8_10