RNA-Sequencing and Transcriptome Analysis after Fluid Shear Stress Stimulation in Lymphatic Endothelial Cells. Chapter uri icon

abstract

  • Fluid shear stress (FSS) is an important mechanical force that regulates endothelial and vascular functions in the blood and lymphatic vasculature. RNA-sequencing (RNA-Seq), an emerging next-generation sequencing (NGS) technique, facilitates us to quantify the global gene expression levels and, thereby, provides us with a powerful tool to study the transcriptional reprograming in the cells. Here, we describe the RNA-Seq procedures and the subsequent bioinformatic analysis to understand the transcriptome regulation by FSS in lymphatic endothelial cells (LECs).

author list (cited authors)

  • Si, H., & Peng, X. u.

complete list of authors

  • Si, Hongjiang||Peng, Xu

Book Title

  • Methods in Molecular Biology

publication date

  • July 2021