RNA-Sequencing and Transcriptome Analysis after Fluid Shear Stress Stimulation in Lymphatic Endothelial Cells. Chapter

abstract

• Fluid shear stress (FSS) is an important mechanical force that regulates endothelial and vascular functions in the blood and lymphatic vasculature. RNA-sequencing (RNA-Seq), an emerging next-generation sequencing (NGS) technique, facilitates us to quantify the global gene expression levels and, thereby, provides us with a powerful tool to study the transcriptional reprograming in the cells. Here, we describe the RNA-Seq procedures and the subsequent bioinformatic analysis to understand the transcriptome regulation by FSS in lymphatic endothelial cells (LECs).

authors

• Peng, Xu

altmetric score

• 0.75

author list (cited authors)

• Si, H., & Peng, X. u.

citation count

• 0

complete list of authors

• Si, Hongjiang||Peng, Xu

Book Title

• Cardiovascular Development

publication date

• January 2021

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Cells, Cultured
• Endothelial Cells
• Fluid Shear Stress
• Gene Expression Profiling
• Humans
• Lymphatic Endothelial Cells
• RNA-seq
• RNA-sequencing
• Stress, Mechanical
• Stress, Physiological
• Transcriptome

PubMed Central ID

• 34331239

Digital Object Identifier (DOI)

• 10.1007/978-1-0716-1480-8_3

International Standard Book Number (ISBN) 13

• 9781071614792

start page

• 25

end page

• 30

volume

• 2319

URL

• http://dx.doi.org/10.1007/978-1-0716-1480-8_3