Analysis of Angiogenesis in Mouse Embryonic Dorsal Skin by Whole-Mount Fluorescent Staining. Chapter uri icon

abstract

  • The blood vascular system is a tree-like hierarchical branching structure and needs to function even before fully established. Abnormal formation of blood vessels results in embryonic lethality and also contributes to the pathogenesis of a number of human diseases, including cancer metastasis. To understand the molecular events associated with blood vessel formation, we established a fluorescence staining-based protocol on mouse embryonic skin. We harvested mouse embryonic skin and performed whole-mount staining. The reconstructed three-dimensional vascular structure provided detailed information onangiogenesis.

author list (cited authors)

  • Wang, J., Dong, Y., Xi, Y. u., & Peng, X. u.

complete list of authors

  • Wang, Jian||Dong, Yuwei||Xi, Yu||Peng, Xu

Book Title

  • Methods in Molecular Biology

publication date

  • July 2021