Analysis of Angiogenesis in Mouse Embryonic Dorsal Skin by Whole-Mount Fluorescent Staining. Chapter

abstract

• The blood vascular system is a tree-like hierarchical branching structure and needs to function even before fully established. Abnormal formation of blood vessels results in embryonic lethality and also contributes to the pathogenesis of a number of human diseases, including cancer metastasis. To understand the molecular events associated with blood vessel formation, we established a fluorescence staining-based protocol on mouse embryonic skin. We harvested mouse embryonic skin and performed whole-mount staining. The reconstructed three-dimensional vascular structure provided detailed information onangiogenesis.

authors

• Peng, Xu

author list (cited authors)

• Wang, J., Dong, Y., Xi, Y. u., & Peng, X. u.

citation count

• 0

complete list of authors

• Wang, Jian||Dong, Yuwei||Xi, Yu||Peng, Xu

Book Title

• Cardiovascular Development

publication date

• January 2021

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Angiogenesis
• Animals
• Dorsal Skin
• Endothelial Cells
• Immunohistochemistry
• Mice
• Microscopy, Confocal
• Microscopy, Fluorescence
• Neovascularization, Physiologic
• Skin
• Staining And Labeling
• Vascular Endothelial Cells
• Whole-mount Staining

PubMed Central ID

• 34331243

Digital Object Identifier (DOI)

• 10.1007/978-1-0716-1480-8_7

International Standard Book Number (ISBN) 13

• 9781071614792

start page

• 61

end page

• 67

volume

• 2319

URL

• http://dx.doi.org/10.1007/978-1-0716-1480-8_7