Alphavirus Purification Using Low-speed Spin Centrifugation. Academic Article uri icon


  • Chemical and sedimentation procedures are used to purify virus particles. While these approaches are successful for wild-type viruses, they are often not feasible for purifying mutant viruses with assembly defects. We combined two published methods ( Atasheva et al., 2013 ; Moller- Tank et al., 2013 ), to generate a protocol that uses low-speed centrifugation to purify both wildtype and mutant enveloped virus particles at high yield with minimal handling steps. This protocol has successfully been used to purify alphavirus particles for imaging and structural studies ( Wang et al., 2015 ; Ramsey et al., 2017 ).

published proceedings

  • Bio Protoc

altmetric score

  • 1

author list (cited authors)

  • Rayaprolu, V., Ramsey, J., Wang, J., & Mukhopadhyay, S.

citation count

  • 3

complete list of authors

  • Rayaprolu, Vamseedhar||Ramsey, Jolene||Wang, Joseph Che-Yen||Mukhopadhyay, Suchetana

publication date

  • March 2018