Deciphering viral infections by ribosome profiling.
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abstract
"Ribosome profiling" combines deep sequencing of RNA and "nuclease footprinting". It is used to monitor all mRNAs and their translation in given cells simultaneously. Ribosome profiling has been shown to be a powerful method for annotating virus genomes due to the discovery of new viral proteins. Also, ribosome profiling can help in the study of translation mechanisms by identifying unknown translation features. Moreover, ribosome profiling can be employed to detect dynamic changes in translation in infected cells, thereby providing novel insights into virus-host interactions. Six DNA viruses and four RNA viruses have been investigated using this method. Here, we summarize the latest applications of ribosome profiling in various viruses to provide insightful information to stimulate further study .