Ribosome Profiling of Vaccinia Virus-Infected Cells. Chapter

abstract

• Ribosome profiling is a method that determines genome-wide mRNA translation through measuring ribosome-protected mRNA fragments by deep sequencing. This method can be used to quantify gene expression at the translational level and precisely pinpoint ribosome loading onto mRNA with codon-level resolution. Genome-wide regulation of mRNA translation can also be determined if RNA-Sequencing (RNA-Seq) is carried out in parallel. Here, we describe a protocol for simultaneously performing ribosome profiling and RNA-Seq in cells infected with vaccinia virus.

authors

• Yang, Zhilong

author list (cited authors)

• Lin, Y., Qiao, W., & Yang, Z.

complete list of authors

• Lin, Yongquan||Qiao, Wentao||Yang, Zhilong

Book Title

• VACCINIA VIRUS

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Gene Expression
• High-Throughput Nucleotide Sequencing
• Humans
• Poxvirus
• Protein Biosynthesis
• RNA, Messenger
• RNA-seq
• Ribosome Profiling
• Ribosomes
• Translation
• Vaccinia Virus

PubMed Central ID

• 31240678

Digital Object Identifier (DOI)

• 10.1007/978-1-4939-9593-6_11

International Standard Book Number (ISBN) 13

• 978-1-4939-9592-9

start page

• 171

end page

• 188

volume

• 2023