Vaccinia Virus Transcriptome Analysis by RNA Sequencing. Chapter

abstract

• RNA-sequencing (RNA-Seq) using next-generation sequencing (NGS) technique is a powerful tool for simultaneous analysis of global transcripts from both vaccinia virus and host cell. Here, we describe an RNA-Seq method for analyzing the vaccinia virus transcriptome from virus-infected HeLa cells. We pay particular attention to vaccinia virus-specific aspects of sample preparation, sequencing, and data analyses, but our method could be modified to analyze transcriptomes of other cells or tissues infected with different poxviruses.

authors

• Yang, Zhilong

author list (cited authors)

• Cao, S., Lin, Y., & Yang, Z.

complete list of authors

• Cao, Shuai||Lin, Yongquan||Yang, Zhilong

Book Title

• VACCINIA VIRUS

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Exome Sequencing
• Gene Expression
• Gene Expression Profiling
• Hela Cells
• High-Throughput Nucleotide Sequencing
• Humans
• Poxvirus
• RNA-seq
• Sequence Analysis, RNA
• Transcriptome
• Vaccinia Virus

PubMed Central ID

• 31240677

Digital Object Identifier (DOI)

• 10.1007/978-1-4939-9593-6_10

International Standard Book Number (ISBN) 13

• 978-1-4939-9592-9

start page

• 157

end page

• 170

volume

• 2023