One-Step Homogeneous Immunoassay for the Detection of Influenza Virus Using Switching Peptide and Graphene Quencher. Academic Article uri icon


  • One-step homogeneous immunoassay was developed for detecting influenza viruses A and B (Inf-A and Inf-B) using the switching peptide H2. As the fluorescence-labeled switching peptide dissociated from the binding pocket of detection antibodies, the fluorescence signal could be directly generated by the binding of Inf-A and Inf-B without washing (i.e., one-step immunoassay). For the one-step homogeneous immunoassay with detection antibodies in solution, graphene was labeled with the antibodies as a fluorescence quencher. To test the feasibility of the homogeneous one-step immunoassay, the stability of the antibody complex with the switching peptide was evaluated under different pH and salt conditions. The one-step homogeneous immunoassay with switching peptide was conducted using influenza virus antigens in phosphate-buffered saline and real samples with inactivated Inf-A and Inf-B spiked in serum. Finally, the one-step homogeneous immunoassay results were compared with those of commercially available lateral flow immunoassays.

published proceedings

  • Biochip J

author list (cited authors)

  • Kim, H., Bong, J., Kim, T., Shin, S., Kang, M., Shim, W., ... Pyun, J.

citation count

  • 1

complete list of authors

  • Kim, Hong-Rae||Bong, Ji-Hong||Kim, Tae-Hun||Shin, Seung-Shick||Kang, Min-Jung||Shim, Won-Bo||Lee, Do Young||Son, Dong Hee||Pyun, Jae-Chul

publication date

  • September 2022