Pseudomonas uvaldensis sp. nov., a bacterial pathogen causing onion bulb rot. Academic Article uri icon

abstract

  • A Gram-stain-negative, aerobic and non-spore-forming bacterial strain, designated 20TX0172T, was isolated from a rotting onion bulb in Texas, USA. The results of phylogenetic analysis based on the 16S rRNA sequence indicated that the novel strain represented a member of the genus Pseudomonas and had the greatest sequence similarities with Pseudomonas kilonensis 520-20T (99.3%), Pseudomonas corrugata CFBP 2431T (99.2%), and Pseudomonas viciae 11K1T (99.2%) but the 16S rRNA phylogenetic tree displayed a monophyletic clade with Pseudomonas mediterranea CFBP 5447T. In the phylogenetic trees based on sequences of four housekeeping genes (gap1, gltA, gyrB and rpoD), the novel strain formed a separate branch, indicating that the strain was distinct phylogenetically from known species of the genus Pseudomonas. The genome-sequence-derived average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the novel isolate and P. mediterranea DSM 16733T were 86.7 and 32.7%, respectively. These values were below the accepted species cutoff threshold of 96% ANI and 70% dDDH, affirming that the strain represented a novel species. The genome size of the novel species was 5.98 Mbp with a DNA G+C content of 60.8mol%. On the basis of phenotypic and genotypic characteristics, strain 20TX0172T represents a novel species of the genus Pseudomonas. The name Pseudomonas uvaldensis sp. nov. is proposed. The type strain is 20TX0172T (=NCIMB 15426T=CIP 112022T).

published proceedings

  • Int J Syst Evol Microbiol

altmetric score

  • 58.45

author list (cited authors)

  • Khanal, M., Timilsina, S., Bhatta, B. P., Bophela, K., Coutinho, T., Cochran, K., & Malla, S.

citation count

  • 4

complete list of authors

  • Khanal, Manzeal||Timilsina, Sujan||Bhatta, Bed Prakash||Bophela, Khumbuzile||Coutinho, Teresa||Cochran, Kimberly||Malla, Subas

publication date

  • January 2022