Optogenetic control of calcium influx in mammalian cells. Chapter

abstract

• Optogenetics combines optics and genetics to enable non-invasive interrogation of cell physiology at an unprecedented high spatiotemporal resolution. Here, we introduce Opto-CRAC as a set of genetically-encoded calcium actuators (GECAs) engineered from the calcium release-activated calcium (CRAC) channel, which has been tailored for optical control of calcium entry and calcium-dependent physiological responses in non-excitable cells and tissues. We describe a detailed protocol for applying Opto-CRAC as an optogenetic tool to achieve photo-tunable control over intracellular calcium signals and calcium-dependent gene expression in mammalian cells.

authors

• Zhou, Yubin

author list (cited authors)

• Lee, Y., Chen, R., Zhou, Y., & He, L.

citation count

• 0

complete list of authors

• Lee, Yi-Tsang||Chen, Rui||Zhou, Yubin||He, Lian

Book Title

• ION CHANNELS: CHANNEL CHEMICAL BIOLOGY, ENGINEERING, AND PHYSIOLOGICAL FUNCTION

publication date

• January 2021

publisher

• Elsevier  Publisher

published in

• METHODS IN ENZYMOLOGY  Journal

keywords

• Actuators
• Animals
• CRISPR
• Calcium
• Calcium Release Activated Calcium Channels
• Calcium Signaling
• Ion Channel
• Optogenetics
• Orai1
• Protein Engineering
• STIM1
• Synthetic Biology
• Transcriptional Programming

PubMed Central ID

• 34120716

Digital Object Identifier (DOI)

• 10.1016/bs.mie.2021.02.010

International Standard Book Number (ISBN) 13

• 9780128239247

start page

• 255

end page

• 270

volume

• 654

URL

• http://dx.doi.org/10.1016/bs.mie.2021.02.010