Dynamics of unfolded nucleosomal fiber. Academic Article

abstract

• The "rigidity" of chromatin fiber solenoidal structure in different states of condensation was evaluated with the help of gel-electrophoresis. A new property of the unfolded nucleosomal fiber-the capacity to condense with temperature-was demonstrated. These results together with our previously obtained data (W.A. Krajewski et al., Mol. Gen. Genet. 230, pp. 442-448, 1991; W.A. Krajewski et al., Ibid. 231, pp. 17-22, 1991) testify that changes in DNA linking number of transcriptionally active minichromosomes arise in vivo from alteration of nucleosomal solenoid parameters (i.e. from supernucleosomal level of chromatin organization), rather than from core histone modifications only or from increased flexibility of DNA within nucleosomes.

authors

• Panin, Vladislav

published proceedings

• J Biomol Struct Dyn

author list (cited authors)

• Krajewski, W. A., Panin, V. M., & Razin, S. V.

citation count

• 6

complete list of authors

• Krajewski, WA||Panin, VM||Razin, SV

publication date

• January 1993

publisher

• Taylor & Francis  Publisher

published in

• Journal of Biomolecular Structure and Dynamics  Journal

keywords

• Animals
• Cell Fractionation
• Chromatin
• DNA
• Electrophoresis, Polyacrylamide Gel
• Histones
• Liver
• Models, Structural
• Nucleic Acid Conformation
• Nucleosomes
• Rats
• Transcription, Genetic

Digital Object Identifier (DOI)

• 10.1080/07391102.1993.10508693

start page

• 1013

end page

• 1022

volume

• 10

issue

• 6

URL

• http://dx.doi.org/10.1080/07391102.1993.10508693