Nanoparticle Assisted Remodeling of Proteotoxic SOD1 Mutants Alters the Biointerface of the Functional Interaction of Microtubules and Kinesin Motors. Academic Article uri icon

abstract

  • Transport deficits with motor neuron degeneration have been implicated in amyotrophic lateral sclerosis (ALS). We report a biomimetic system composed of microtubules/kinesin motor that mimics the dysregulated motor dynamics of ALS. Pathogenic ALS mutants A4V SOD1 completely shut off motility. Treatment with 5 nm citrate coated gold nanoparticles recovers the impaired motor stepping by remodeling the A4V SOD1 rather than stabilizing microtubules or protein folding. Instead, gold nanoparticles alter the protein by a mechanism that reforms protein elements of A4V SOD1, in turn fixing the aberrant interaction of kinesin with microtubules. Reinstating kinesin motility holds potential for managing debilitating ALS.

published proceedings

  • ACS Appl Bio Mater

author list (cited authors)

  • Kim, K., Subramaniyam, S., Galaleldeen, A., Nakazawa, H., Umetsu, M., Teizer, W., & Bhattacharyya, S.

citation count

  • 3

complete list of authors

  • Kim, Kyongwan||Subramaniyam, Selvaraj||Galaleldeen, Ahmad||Nakazawa, Hikaru||Umetsu, Mitsuo||Teizer, Winfried||Bhattacharyya, Sanjib

publication date

  • January 2019