Single-beam multimodal nonlinear-optical imaging of structurally complex events in cell-cycle dynamics Academic Article uri icon

abstract

  • Abstract We demonstrate a multimodal nonlinear-optical imaging that combines second- and third-harmonic generation (SHG and THG) with three-photon-excited fluorescence (3PEF) as a means to resolve fine details of the cell structure and trace its transformations throughout structurally complex episodes of cell-cycle dynamics, including the key stages and signatures in cell division. When zoomed in on cell mitosis, this technique enables a high-contrast multimodal imaging of intra- and extracellular signatures of cell division, detecting, via a multiplex, 3PEF/SHG/THG readout, a remarkable diversity of shapes, sizes, and symmetries in a truly single-beam setting, with no need for beam refocusing or field-waveform re-adjustment.

published proceedings

  • JOURNAL OF PHYSICS-PHOTONICS

author list (cited authors)

  • Lanin, A. A., Chebotarev, A. S., Kelmanson, I. V., Pochechuev, M. S., Fetisova, E. S., Bilan, D. S., ... Zheltikov, A. M.

citation count

  • 4

complete list of authors

  • Lanin, AA||Chebotarev, AS||Kelmanson, IV||Pochechuev, MS||Fetisova, ES||Bilan, DS||Shevchenko, EK||Ivanov, AA||Fedotov, AB||Belousov, VV||Zheltikov, AM

publication date

  • October 2021