Adaptive Wave-Front Shaping and Beam Focusing through Fiber Bundles for High-Resolution Bioimaging Academic Article uri icon

abstract

  • We demonstrate an adaptive wave-front shaping of optical beams transmitted through fiber bundles as a powerful resource for multisite, high-resolution bioimaging. With the phases of all the beamlets delivered through up to 6000 different fibers within the fiber bundle controlled individually, by means of a high-definition spatial light modulator, the overall beam transmitted through the fiber bundle can be focused into a beam waist with a diameter less than 1 m within a targeted area in a biotissue, providing a diffraction-limited spatial resolution adequate for single-cell or even subcellular bioimaging. The field intensity in the adaptively-focused continuous-wave laser beam in our fiber-bundle-imaging setting is more than two orders of magnitude higher than the intensity of the speckle background. Once robust beam focusing was achieved with a suitable phase profile across the input face of the fiber bundle, the beam focus can be scanned over a targeted area with no need for a further adaptive search, by applying a physically intuitive, wave-front-tilting phase mask on the field of input beamlets. This method of beam-focus scanning promises imaging speeds compatible with the requirements of in vivo calcium imaging.

published proceedings

  • PHOTONICS

author list (cited authors)

  • Pochechuev, M. S., Fedotov, I. V., Solotenkov, M. A., Andreeva, M. S., Lanin, A. A., Fedotov, A. B., & Zheltikov, A. M.

citation count

  • 1

complete list of authors

  • Pochechuev, Matvey S||Fedotov, Ilya V||Solotenkov, Maxim A||Andreeva, Maria S||Lanin, Aleksandr A||Fedotov, Andrei B||Zheltikov, Aleksei M

publication date

  • December 2022

publisher