Dissection of Race 1 Anthracnose Resistance in a Watermelon (Citrullus lanatus var. lanatus) Biparental Mapping Population Institutional Repository Document uri icon


  • Abstract Anthracnose, caused by the fungal pathogen Colletotrichum orbiculare (Berk. & Mont.) Arx syn. lagenaria, is one of the most important diseases of watermelon in the United States and worldwide. The study was conducted to identify C. orbiculare race 1 resistance quantitative trait loci (QTL) in a Charleston Gray, resistant parent, and New Hampshire Midget, susceptible parent, biparental mapping population. The mapping population consisted of 228 F2 and the validation population consisted of 60 individuals each in BC1P1 and BC1P2. The disease severity was rated using a disease index comprising a rating scale of 0 to 100%. IciMapping was used to draw the linkage map and R/qtl non-parametric method (model = np) was used to identity QTL. We identified a major disease resistance QTL, Qar1-8, on chromosome 8. The significant SNP marker S8_5149002, part of a putative coiled-coil (CC)nucleotide-binding site (NBS)leucine-rich repeat (LRR) (CC-NBS-LRR or CNL; ClCG08G002410), had a LOD of 14.06. The significant marker was validated on mapping populations using R package functions chisq.test, wilcox.test, kruskal.test, and dunn.test. The significant marker S8_5149002 was also tested for its ability to differentiate race 1 anthracnose resistance on 61 watermelon germplasm including 41 plant introduction (PI) lines. Hence, the diagnostic SNP marker S8_5149002 could be used for marker assisted selection (MAS) for race 1 anthracnose resistance in watermelon breeding programs.

altmetric score

  • 1

author list (cited authors)

  • Bhatta, B. P., Patel, T., Correa, E., Wehner, T. C., Crosby, K. M., Thomson, M. J., ... Malla, S.

citation count

  • 0

complete list of authors

  • Bhatta, Bed Prakash||Patel, Takshay||Correa, Edgar||Wehner, Todd C||Crosby, Kevin M||Thomson, Michael J||Metz, Richard||Wang, Shichen||Brun, Marcel||Johnson, Charles D||Malla, Subas

Book Title

  • Research Square

publication date

  • June 2022