Mechanistic Studies on the Single-Turnover Yeast Thiamin Pyrimidine Synthase: Characterization of the Inactive Enzyme. Academic Article uri icon


  • The eukaryotic thiamin pyrimidine synthase, THI5p, has been identified as a suicidal/single-turnover enzyme that catalyzes the conversion of its active site histidine and lysine-bound pyridoxal phosphate (PLP) to the thiamin pyrimidine (HMP-P). Here we identify the histidine and PLP fragments using bottom-up proteomics and LC-MS analysis. We also identify the active form of the iron cofactor and quantitate the oxygen requirement of the THI5p reaction. This information is integrated into a mechanistic proposal for this remarkable reaction.

published proceedings

  • J Am Chem Soc

author list (cited authors)

  • Lai, R., Mondal, A., Fedoseyenko, D., & Begley, T. P.

complete list of authors

  • Lai, Rung-Yi||Mondal, Anushree||Fedoseyenko, Dmytro||Begley, Tadhg P

publication date

  • January 1, 2022 11:11 AM