Lipopolysaccharide activates endothelial nitric oxide synthase through protein tyrosine kinase. Academic Article

abstract

• Vascular endothelial cell injury or activation by lipopolysaccharide (LPS) plays an important role in the pathogenesis of endotoxin shock. However, the effect of LPS on NO production from vascular endothelial cells (ECs) is incompletely understood. In this study, bovine coronary venular ECs were treated with LPS and the release of NO and expression of the endothelial NO synthase (ecNOS) were examined. We found that the ecNOS activity is transiently enhanced by LPS within the time scale of about 10 h due to the interplay between two LPS-induced mechanisms. Within the first 10 h of LPS treatment, the specific activity of ecNOS is increased by a post-translational modification mediated through a protein tyrosine kinase cascade. After about 10 h of treatment, however, LPS destabilizes the transcript of ecNOS and thus decreased the expression level and total activity.

authors

• Kuo, Lih

published proceedings

• Biochem Biophys Res Commun

author list (cited authors)

• Huang, K. T., Kuo, L., & Liao, J. C.

citation count

• 26

complete list of authors

• Huang, KT||Kuo, L||Liao, JC

publication date

• January 1998

publisher

• Elsevier  Publisher

published in

• Biochemical and Biophysical Research Communications  Journal

keywords

• Animals
• Bradykinin
• Calcimycin
• Cattle
• Cells, Cultured
• Down-Regulation
• Endothelium, Vascular
• Enzyme Activation
• Enzyme Inhibitors
• Genistein
• Ionophores
• Lipopolysaccharides
• Muscle, Smooth, Vascular
• Nitric Oxide
• Nitric Oxide Synthase
• Protein Processing, Post-Translational
• Protein-Tyrosine Kinases
• Shock, Septic

Digital Object Identifier (DOI)

• 10.1006/bbrc.1998.8384

start page

• 33

end page

• 37

volume

• 245

issue

• 1

URL

• http://dx.doi.org/10.1006/bbrc.1998.8384