Cetylpyridinium chloride inhibits human breast tumor cells growth in a no-selective way. Academic Article uri icon

abstract

  • OBJECTIVE: Analyze the antitumor capacity of cetylpyridinium chloride (CPC) on human breast tumor cells, and the possible action mechanism. MATERIAL AND METHODS: The human breast tumor cells MCF-7 and no-tumor breast cells MCF-10A were exposed to CPC under various condition (concentration and duration). Cell viability was measured with MTT assay, the LIVE/DEAD assay, and fluorescence microscopy. Membrane permeability after CPC exposure was evaluated by Calcein AM assay, mitochondrial morphology with a MitoView staining, and genotoxicity with the comet assay and fluorescence microscopy. RESULTS: CPC was cytotoxic to both MCF-7 and MCF-10A as of a 24-h exposure to 0.1M. Cytotoxicity was dose-dependent and reached 91% for MCF-7 and 78% for MCF-10A after a 24-h exposure to 100M CPC, which outperformed the positive control doxorubicin in effectiveness and selectivity. The LD50 of CPC on was 6M for MCF-7 and 8M for MCF-10A, yielding a selectivity index of 1.41. A time response analysis revealed 64% dead cells after only 5min of exposure to 100M CPC. With respect to the action mechanisms, the comet assay did not reveal genome fragmentation. On the other hand, membrane damage was dose-dependent and may also affect mitochondrial morphology. CONCLUSION: Cetylpyridinium chloride inhibits MCF-7 cell growing in a non-selective way as of 5min of exposure. The action mechanism of CPC on tumor cells involves cell membrane damage without change neither mitochondrial morphology nor genotoxicity.

published proceedings

  • J Appl Biomater Funct Mater

author list (cited authors)

  • Garca-Cuellar, C. M., Hernndez-Delgadillo, R., Solis-Soto, J. M., Meester, I., Snchez-Prez, Y., Nakagoshi-Cepeda, S. E., ... Cabral-Romero, C.

complete list of authors

  • García-Cuellar, Claudia María||Hernández-Delgadillo, Rene||Solis-Soto, Juan Manuel||Meester, Irene||Sánchez-Pérez, Yesennia||Nakagoshi-Cepeda, Sergio Eduardo||Nakagoshi-Cepeda, María Argelia Akemi||Chellam, Shankararaman||Cabral-Romero, Claudio

publication date

  • January 2022