A chemifluorescent immunoassay for the determination of marinobufagenin in body fluids.
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We describe here the development of a chemifluorescent competitive enzyme-linked immunosorbent assay (ELISA) that quantifies marinobufagenin (MBG) levels in biological fluids. Based on a polyclonal antibody raised against a novel MBG-bovine serum albumin conjugate, this assay achieved an MBG detection limit of less than 9 pg/mL. MBG levels in various rat urine and serum samples were effectively determined using this methodology. Interassay variability averaged 9.8%, while intra-assay variability averaged 1.9 and 2.5% in representative serum and urine samples, respectively. Recovery of exogenously added MBG averaged 106%, and parallelism data further established the accuracy of the assay. Employment of this assay to detect MBG abnormalities represents a powerful tool for the possible diagnosis, prevention and management of human hypertensive states, particularly preeclampsia.
author list (cited authors)
Abi-Ghanem, D., Lai, X., Berghman, L. R., Horvat, D., Li, J., Romo, D., ... Puschett, J. B.
complete list of authors
Abi-Ghanem, Daad||Lai, Xinzhong||Berghman, Luc R||Horvat, Darijana||Li, Jing||Romo, Daniel||Uddin, Mohammad N||Kamano, Yoshiaki||Nogawa, Toshihiko||Xu, Jun-Ping||Pettit, George R||Puschett, Jules B