Differential effects of virulent and avirulent equine infectious anemia virus on macrophage cytokine expression. Academic Article

abstract

• Equine infectious anemia virus (EIAV) causes rapid development of acute disease followed by recurring episodes of fever, thrombocytopenia, and viremia. Most infected equid eventually bring the virus under immunological control. We recently reported the development of an equine-specific ribonuclease protection assay (RPA) to quantitate mRNA levels of 10 cytokines. Using this newly developed RPA, we now show significant differences in cytokine induction in equine monocyte-derived macrophages (EMDM) exposed to virulent and avirulent EIAV. Virulent EIAV17 induced significant increases in interleukin (IL)-1alpha, IL-1beta, IL-6, IL-10, and tumor necrosis factor (TNF)-alpha by 0.5-1 h postinfection (hpi). In contrast, the avirulent virus failed to induce any of the tested cytokines above that of control levels. These data show a direct correlation between cytokine dysregulation and EIAV pathogenesis.

authors

• Edwards, John
• Payne, Susan

published proceedings

• Virology

author list (cited authors)

• Lim, W., Payne, S. L., Edwards, J. F., Kim, I., & Ball, J. M.

citation count

• 10

complete list of authors

• Lim, W-S||Payne, SL||Edwards, JF||Kim, I||Ball, JM

publication date

• February 2005

publisher

• Elsevier  Publisher

published in

• Virology  Journal

keywords

• Animals
• Cells, Cultured
• Cytokines
• Gene Expression Regulation, Viral
• Infectious Anemia Virus, Equine
• Leukocytes, Mononuclear
• Macrophages
• RNA, Messenger
• Ribonucleases
• Tumor Necrosis Factor-alpha
• Virulence

Digital Object Identifier (DOI)

• 10.1016/j.virol.2004.11.027

start page

• 295

end page

• 306

volume

• 332

issue

• 1

URL

• http://dx.doi.org/10.1016/j.virol.2004.11.027

user-defined tag

• 3 Good Health and Well-Being