Validation of ultrasonography for measurement of cartilage thickness in the equine carpus. Academic Article uri icon


  • Articular cartilage thinning is an important hallmark of osteoarthritis (OA), and ultrasonography (US) is a clinically accessible tool potentially suitable for repeated evaluation. The aim of the present prospective methods comparison study was to validate US as a tool for measuring cartilage thickness in the carpus of the horse. Eight Standardbred trotters underwent US examination with 9 and 15MHz linear transducers. Six anatomical locations in the radiocarpal joint (RCJ) and middle carpal joint (MCJ) were examined. The same joints were assessed by ultrahigh field (9.4 Tesla) magnetic resonance imaging (MRI) and histology. Associations between measurements obtained by the different modalities were assessed by ANOVA, Deming regression, Pearson correlation and Bland-Altman plots. Histologically assessed total cartilage thickness (the noncalcified cartilage (NCC) plus the calcified cartilage zone (CCZ)) overestimated thickness compared to MRI (P<0.01) and US (P<0.01). US 15MHz had substantial agreement with MRI and NCC histology, and repeatability was acceptable (coefficient of variation=8.6-17.9%) when used for assessment of cartilage thickness in the RCJ. In contrast, 9MHz US showed poorer agreement with MRI and NCC histology, as it overestimated the thickness of thin cartilage and underestimated the thickness of thicker cartilage in the RCJ and MCJ. Moreover, repeatability was suboptimal (coefficient of variation=10.4-26.3%). A 15MHz transducer US is recommended for detecting changes in RCJ cartilage thickness or monitoring development over time, and it has the potential for noninvasive assessment of cartilage health in horses.

published proceedings

  • Vet Radiol Ultrasound

author list (cited authors)

  • Andersen, C., Griffin, J. F., Jacobsen, S., stergaard, S., Walters, M., Mori, Y., & Lindegaard, C.

citation count

  • 0

complete list of authors

  • Andersen, Camilla||Griffin, John F||Jacobsen, Stine||Ƙstergaard, Stine||Walters, Marie||Mori, Yuki||Lindegaard, Casper

publication date

  • January 2022