Shin, Young J. (2009-05). Amperometric Glucose Biosensor by Means of Electrostatic Layer-by-layer Adsorption onto Electrospun Polyaniline Fibers. Master's Thesis. Thesis uri icon

abstract

  • An amperometric glucose biosensor was fabricated using electospun polyaniline fibers. Polyaniline was reacted with camphorsulfonic acid to produce a salt, which was then dissolved in chloroform containing polystyrene. Using this solution, fibers were formed and collected by electrospinning. Glucose oxidase was immobilized onto these fibers using an electrostatic layer-by-layer adsorption technique. In this method, poly(diallyldimethylammonium chloride) was used as the counter ion source. The level of adsorption was examined and evidence of layer-by-layer adsorption was obtained using a quartz crystal microbalance technique. A biosensor was fabricated from these fibers as a working electrode, and used to measure the glucose concentration accurately.
  • An amperometric glucose biosensor was fabricated using electospun polyaniline
    fibers. Polyaniline was reacted with camphorsulfonic acid to produce a salt, which was
    then dissolved in chloroform containing polystyrene. Using this solution, fibers were
    formed and collected by electrospinning. Glucose oxidase was immobilized onto these
    fibers using an electrostatic layer-by-layer adsorption technique. In this method,
    poly(diallyldimethylammonium chloride) was used as the counter ion source. The level
    of adsorption was examined and evidence of layer-by-layer adsorption was obtained
    using a quartz crystal microbalance technique. A biosensor was fabricated from these
    fibers as a working electrode, and used to measure the glucose concentration accurately.

publication date

  • May 2009