Ionizing radiation which results in the free radical formation and it leads to damage of biological macromolecules such as DNA, proteins, lipids. 36 male Swiss albino mice were used for survival assay, to find out the lethal dose of Electron Beam Radiation. It was found to be 10Gy was the lethal dose for mice. Different dosages (4Gy, 6Gy and 8Gy) of electron beam radiation were used to study the micronucleus formation in irradiated mice. The results showed micronucleus formation will increase linearly with radiation dosage.