Analytical validation of a novel point-of-care immunoassay for canine N-terminal pro-brain natriuretic peptide analysis. Academic Article uri icon


  • BACKGROUND: N-terminal pro-brain natriuretic peptide (NT-proBNP) is a widely used point-of-care (POC) cardiac biomarker in human medicine. Canine NT-proBNP is used less in veterinary medicine, possibly due to the lack of a POC canine NT-proBNP assay resulting in temporal delay, increased degradation in transport, and high reported variability in the available assay. A new quantitative POC analyzer allows fast, onsite measurement of NT-proBNP, minimizing preanalytical error and reducing variability. OBJECTIVE: We aimed to analytically validate an NT-proBNP assay (Vcheck) according to American Society of Veterinary Clinical Pathology (ASVCP) and Clinical Laboratory Improvement Amendments (CLIA) specifications. METHODS: Archived and prospective plasma and serum samples were collected from male and female, client-owned dogs of various breeds with cardiac abnormalities (n=81) and a healthy control population (n=225). Precision, accuracy, analytical sensitivity, and specificity, and other statistical analyses were performed. RESULTS: Imprecision was considered acceptable with a coefficient of variation ranging from 9% at 4000pmol/L to 20% at 600pmol/L. The lower limit of quantitation was 650pmol/L based on repetitive measures evaluation. Comparison of the Vcheck assay with the Cardiopet NT-proBNP assay revealed an excellent correlation with minimal bias when preanalytical factors were controlled. Significant degradation of NT-proBNP occurred when current methods were used at refrigerated and room temperatures, which could change diagnostic and prognostic decision-making. Age-partitioned reference intervals have high reference values of 750pmol/L and 1280pmol/L for juvenile and adult dogs, respectively. CONCLUSIONS: The Vcheck NT-ProBNP assay provides analytically acceptable results. Onsite testing can minimize variability related to preanalytical error and provide clinically useful contemporaneous results. Samples should be centrifuged immediately and analyzed within 2 hours of collection.

published proceedings

  • Vet Clin Pathol

altmetric score

  • 0.25

author list (cited authors)

  • Harr, K. E., Gordon, S. G., Baumwart, R. D., Feldgreber, R., & Spiro, M. R.

citation count

  • 2

complete list of authors

  • Harr, Kendal E||Gordon, Sonya G||Baumwart, Ryan D||Feldgreber, Ross||Spiro, Matthew R

publication date

  • January 2022