Hydrophobic and Hydrogel-Based Methods for Passive Tissue Clearing. Chapter uri icon


  • Optical tissue clearing enables the precise imaging of cellular and subcellular structures in whole organs and tissues without the need for physical tissue sectioning. By combining tissue clearing with confocal or lightsheet microscopy, 3D images can be generated of entire specimens for visualization and large-scale data analysis. Here we demonstrate two different passive tissue clearing techniques that are compatible with immunofluorescent staining and lightsheet microscopy: PACT, an aqueous hydrogel-based clearing method, and iDISCO+, an organic solvent-based clearing method.

author list (cited authors)

  • Jalufka, F. L., Min, S. W., Platt, M. E., Pritchard, A. L., Margo, T. E., Vernino, A. O., ... McCreedy, D. A.

complete list of authors

  • Jalufka, Frank L||Min, Sun Won||Platt, Madison E||Pritchard, Anna L||Margo, Theodore E||Vernino, Alexandra O||Kirchhoff, Megan A||Massopust, Ryan T||McCreedy, Dylan A

Book Title

  • Methods in Molecular Biology

publication date

  • February 2022