Effects of progesterone and interferon tau on ovine endometrial phosphate, calcium, and vitamin D signaling.
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Given recent reports of expression of postnatal mineral transport regulators at the maternal-conceptus interface during the peri-implantation period, this study tested the hypothesis that progesterone (P4) and interferon tau (IFNT) regulate phosphate, calcium, and vitamin D signaling in the ovine endometrium. Mature Rambouillet ewes (n=24) were surgically fitted with intrauterine catheters on day 7 of the estrous cycle. Ewes received daily intramuscular injections of 50mg of P4 in corn oil vehicle and 75mg of progesterone receptor antagonist (RU486) in corn oil from days 8 to 15, and twice-daily intrauterine injections of either control proteins (CX) or IFNT (25g/uterine horn/day) from days 11 to 15 resulting in four treatment groups: P4+CX; P4+IFNT; RU486+P4+CX; and RU486+P4+IFNT. On day 16, ewes were hysterectomized. RU486+P4+CX treated ewes had lower concentrations of 25 (OH) D in plasma than P4+CX treated ewes (P<0.05). Endometria from ewes treated with IFNT had greater expression of FGF23 (P<0.01), S100A9 (P<0.05), and S100A12 (P=0.05) mRNAs and lower expression of ADAM10 mRNA (P<0.01) than of ewes treated with CX proteins. Expression of FGF23 mRNA was greater in endometria of ewes that received RU486+P4+IFNT than in ewes that received RU486+P4+CX (hormone protein interaction, P<0.05). The expression of S100G mRNA was greater in endometria of ewes that received P4+IFNT compared to ewes that received RU486+P4+IFNT (P<0.05; hormone protein interaction, P<0.01). These data implicate P4 and IFNT in the regulation of phosphate, calcium, and vitamin D signaling during the peri-implantation period of pregnancy and provide a platform for continued mechanistic investigations.
author list (cited authors)
Stenhouse, C., Halloran, K. M., Moses, R. M., Seo, H., Gaddy, D., Johnson, G. A., ... Bazer, F. W.
complete list of authors
Stenhouse, Claire||Halloran, Katherine M||Moses, Robyn M||Seo, Heewon||Gaddy, Dana||Johnson, Gregory A||Wu, Guoyao||Suva, Larry J||Bazer, Fuller W