Effects of progesterone and interferon tau on ovine endometrial phosphate, calcium, and vitamin D signaling.
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abstract
Given recent reports of expression of postnatal mineral transport regulators at the maternal-conceptus interface during the peri-implantation period, this study tested the hypothesis that progesterone (P4) and interferon tau (IFNT) regulate phosphate, calcium, and vitamin D signaling in the ovine endometrium. Mature Rambouillet ewes (n=24) were surgically fitted with intrauterine catheters on day 7 of the estrous cycle. Ewes received daily intramuscular injections of 50mg of P4 in corn oil vehicle and 75mg of progesterone receptor antagonist (RU486) in corn oil from days 8 to 15, and twice-daily intrauterine injections of either control proteins (CX) or IFNT (25g/uterine horn/day) from days 11 to 15 resulting in four treatment groups: P4+CX; P4+IFNT; RU486+P4+CX; and RU486+P4+IFNT. On day 16, ewes were hysterectomized. RU486+P4+CX treated ewes had lower concentrations of 25 (OH) D in plasma than P4+CX treated ewes (P<0.05). Endometria from ewes treated with IFNT had greater expression of FGF23 (P<0.01), S100A9 (P<0.05), and S100A12 (P=0.05) mRNAs and lower expression of ADAM10 mRNA (P<0.01) than of ewes treated with CX proteins. Expression of FGF23 mRNA was greater in endometria of ewes that received RU486+P4+IFNT than in ewes that received RU486+P4+CX (hormone protein interaction, P<0.05). The expression of S100G mRNA was greater in endometria of ewes that received P4+IFNT compared to ewes that received RU486+P4+IFNT (P<0.05; hormone protein interaction, P<0.01). These data implicate P4 and IFNT in the regulation of phosphate, calcium, and vitamin D signaling during the peri-implantation period of pregnancy and provide a platform for continued mechanistic investigations.
