Validation of terminal Schwann cell gene marker expression by fluorescent in situ hybridization using RNAscope. Academic Article

abstract

• Recent RNA-seq studies have generated a new crop of putative gene markers for terminal Schwann cells (tSCs), non-myelinating glia that cap axon terminals at the vertebrate neuromuscular junction (NMJ). While compelling, these studies did not validate the expression of the novel markers using in situ hybridization techniques. Here, we use RNAscope technology to study the expression of top candidates from recent tSC and non-myelinating Schwann cell marker RNA-seq studies. Our results validate the expression of these markers at tSCs but also demonstrate that they are present at other sites in the muscle tissue, specifically, at muscle spindles and along intramuscular nerves.

authors

• Rimer, Mendell

published proceedings

• Neurosci Lett

altmetric score

• 2.85

author list (cited authors)

• Seaberg, B. L., Purao, S., & Rimer, M.

citation count

• 2

complete list of authors

• Seaberg, Bonnie L||Purao, Sohum||Rimer, Mendell

publication date

• January 2022

publisher

• Elsevier  Publisher

published in

• NEUROSCIENCE LETTERS  Journal

keywords

• Animals
• Female
• In Situ Hybridization, Fluorescence
• Male
• Mice
• Mice, Inbred C57BL
• Muscle Spindle
• Nerve Tissue Proteins
• Neuromuscular Junction
• Non-myelinating Schwann Cell
• RNA-seq
• Reference Standards
• Rnascope
• Schwann Cells
• Terminal Schwann Cell

PubMed Central ID

• 35065247

Digital Object Identifier (DOI)

• 10.1016/j.neulet.2022.136468

start page

• 136468

end page

• 136468

volume

• 771

URL

• http://dx.doi.org/10.1016/j.neulet.2022.136468