Aconitine induces cell apoptosis via mitochondria and death receptor signaling pathways in hippocampus cell line. Academic Article

abstract

• Aconitine is a plant toxin derived from aconitum genus and well known for its neurological and vascular toxicity. However, the mechanism of toxicity on the growth and apoptosis of the neurological cells has not been well investigated. In this study, we used HT22 cell lines derived from hippocampus to explore the mechanism. We began with examination of the viability and DA (dopamine) contents of cells treated with different dose of aconitine. In this study, we investigated the role of apoptosis in AC-induced HT22 cells. Our results showed that aconitine inhibited HT22 cells growth and increased DA contents in a dose dependent manner. Aconitine treatment induced apoptosis in HT22 cells and we found aconitine induced apoptosis by upregulating the expression of Bax, Cyto c, Apaf-1, Caspase9, Fas, Fas-L, Fadd, Caspase8, Caspase3 with concomitant decreasing of Bcl-2 and Bid expression. Collectively, results suggest that aconitine induce apoptosis through mitochondrial-mediated and death receptor signaling pathways in HT22 cells.

authors

• Tian, Yanan

published proceedings

• Res Vet Sci

altmetric score

• 0.25

author list (cited authors)

• Wang, H., Liu, Y., Guo, Z., Wu, K., Zhang, Y., Tian, Y., Zhao, B., & Lu, H.

citation count

• 3

complete list of authors

• Wang, Hui||Liu, Yanbing||Guo, Ziyu||Wu, Kexin||Zhang, Yunhao||Tian, Yanan||Zhao, Baoyu||Lu, Hao

publication date

• January 2022

publisher

• Elsevier  Publisher

published in

• Research in Veterinary Science  Journal

keywords

• Aconitine
• Aconitum
• Animals
• Apoptosis
• Apoptotic Pathway
• Cell Apoptosis
• Cell Line
• Fas Receptor
• Hippocampus
• Ht22 Cells
• Mitochondria
• Receptors, Death Domain
• Signal Transduction

Digital Object Identifier (DOI)

• 10.1016/j.rvsc.2022.01.001

start page

• 124

end page

• 133

volume

• 143

URL

• http://dx.doi.org/10.1016/j.rvsc.2022.01.001