Method of Increasing Recovery of Heterologous Active Enzymes Produced in Plants Patent uri icon

abstract

  • A method of increasing recovery of active enzyme produced in a plant is provided where the enzyme requires a transitional metal cofactor for activation. The metal cofactor is supplied to the enzyme during plant development, during extraction, or after extraction. Recovery of active enzyme is also provided by incubating the extracted enzyme at a non-enzyme degrading temperature. Addition of a negative ion salt further improves active enzyme recovery. Optimum salt concentrations for recovery of laccase from plants using copper solutions is provided.

author list (cited authors)

  • Hood, E., Howard, J. A., Bailey, M., van Gastel, F., Ward, M., Wang, H., & Woodard, S.

complete list of authors

  • Hood, E||Howard, JA||Bailey, M||van Gastel, FJC||Ward, M||Wang, H||Woodard, S

publication date

  • October 2003