Rapid generation of random mutant libraries. Academic Article

abstract

• A simple and efficient method utilizing in vivo recombination to create recombinant libraries incorporating the products of PCR amplification is described. This will be especially useful for generating large pools of randomly mutagenized clones after error-prone PCR mutagenesis. Here we investigate various parameters to optimize this approach and we demonstrate that as little as 1 pmole of PCR fragment can generate a library with greater than 104 clones in a single transformation without ligation.

authors

• Benedik, Michael

published proceedings

• Bioeng Bugs

altmetric score

• 4.5

author list (cited authors)

• Abou-Nader, M., & Benedik, M. J.

citation count

• 11

complete list of authors

• Abou-Nader, Mary||Benedik, Michael J

publication date

• January 2010

publisher

• Taylor & Francis  Publisher

published in

• Bioengineered  Journal

keywords

• Bacillus
• Directed Evolution
• Error Prone Pcr
• Gene Library
• In Vivo Cloning
• Lambda Red Proteins
• Mutagenesis
• Mutant Libraries
• Mutation
• Plasmids
• Polymerase Chain Reaction
• Transformation, Genetic

Digital Object Identifier (DOI)

• 10.4161/bbug.1.5.12942

start page

• 337

end page

• 340

volume

• 1

issue

• 5

URL

• http://dx.doi.org/10.4161/bbug.1.5.12942