Soluble antigens derived from Coxiella burnetii elicit protective immunity in three animal models without inducing hypersensitivity
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Q fever is caused by the intracellular bacterium Coxiella burnetii, for which there is no approved vaccine in the United States. A formalin-inactivated whole-cell vaccine (WCV) from virulent C. burnetii NMI provides single-dose long-lived protection, but concerns remain over vaccine reactogenicity. We therefore sought an alternate approach by purifying native C. burnetii antigens from the clonally derived avirulent NMII strain. A soluble bacterial extract, termed Sol II, elicits high-titer, high-avidity antibodies and induces a CD4 T cell response that confers protection in naive mice. In addition, Sol II protects against pulmonary C. burnetii challenge in three animal models without inducing hypersensitivity. An NMI-derived extract, Sol I, enhances protection further and outperforms the WCV gold standard. Collectively, these data represent a promising approach to design highly effective, non-reactogenic Q fever vaccines.
author list (cited authors)
Gregory, A. E., van Schaik, E. J., Fratzke, A. P., Russell-Lodrigue, K. E., Farris, C. M., & Samuel, J. E.
complete list of authors
Gregory, Anthony E||van Schaik, Erin J||Fratzke, Alycia P||Russell-Lodrigue, Kasi E||Farris, Christina M||Samuel, James E