Implementing Digital-Waveform Technology for Extended m/z Range Operation on a Native Dual-Quadrupole FT-IM-Orbitrap Mass Spectrometer. Academic Article uri icon


  • Here, we describe a digital-waveform dual-quadrupole mass spectrometer that enhances the performance of our drift tube FT-IMS high-resolution Orbitrap mass spectrometer (MS). The dual-quadrupole analyzer enhances the instrument capabilities for studies of large protein and protein complexes. The first quadrupole (q) provides a means for performing low-energy collisional activation of ions to reduce or eliminate noncovalent adducts, viz., salts, buffers, detergents, and/or endogenous ligands. The second quadrupole (Q) is used to mass-select ions of interest for further interrogation by ion mobility spectrometry and/or collision-induced dissociation (CID). Q is operated using digital-waveform technology (DWT) to improve the mass selection compared to that achieved using traditional sinusoidal waveforms at floated DC potentials (>500 V DC). DWT allows for increased precision of the waveform for a fraction of the cost of conventional RF drivers and with readily programmable operation and precision (Hoffman, N. M. . A comparison-based digital-waveform generator for high-resolution duty cycle. Review of Scientific Instruments 2018, 89, 084101).

published proceedings

  • J Am Soc Mass Spectrom

altmetric score

  • 3.6

author list (cited authors)

  • McCabe, J. W., Jones, B. J., Walker, T. E., Schrader, R. L., Huntley, A. P., Lyu, J., ... Russell, D. H.

citation count

  • 1

complete list of authors

  • McCabe, Jacob W||Jones, Benjamin J||Walker, Thomas E||Schrader, Robert L||Huntley, Adam P||Lyu, Jixing||Hoffman, Nathan M||Anderson, Gordon A||Reilly, Peter TA||Laganowsky, Arthur||Wysocki, Vicki H||Russell, David H

publication date

  • December 2021