Engineering threshold-based selection systems. Academic Article uri icon


  • Using model organisms to identify novel therapeutic targets is frequently constrained by pre-existing genetic toolkits. To expedite positive selection for identification of novel downstream effectors, we engineered conditional expression of activated CED-10/Rac to disrupt Caenorhabditis elegans embryonic morphogenesis, titrated to 100% lethality. The strategy of engineering thresholds for positive selection using experimental animals was validated with pharmacological and genetic suppression and is generalizable to diverse molecular processes and experimental systems.

published proceedings

  • G3 (Bethesda)

altmetric score

  • 1.25

author list (cited authors)

  • Pedone, K. H., Gonzlez-Prez, V., Leopold, L. E., Rasmussen, N. R., Der, C. J., Cox, A. D., Ahmed, S., & Reiner, D. J.

citation count

  • 0

complete list of authors

  • Pedone, Katherine H||González-Pérez, Vanessa||Leopold, Luciana E||Rasmussen, Neal R||Der, Channing J||Cox, Adrienne D||Ahmed, Shawn||Reiner, David J

publication date

  • September 2021