Pharmacokinetics of long-term low-dose oral rapamycin in four healthy middle-aged companion dogs Institutional Repository Document uri icon

abstract

  • AbstractObjectiveTo determine the blood concentration and pharmacokinetic parameters of rapamycin in companion dogs following long-term, low-dose oral administration of rapamycin.AnimalsFour healthy, middle-aged, medium-to-large breed privately owned dogs participated.ProceduresAll dogs had been receiving oral rapamycin at a dose of 0.025 mg/kg on Monday, Wednesday, and Friday mornings for at least one month. An initial blood sample was collected prior to morning rapamycin administration, and samples were collected at 1, 2, 6, and 24 hours after rapamycin was given. Blood samples were transferred to blood spot collection cards, air-dried and stored at 80C. Rapamycin concentrations were determined via HPLC/MS. All blood collections occurred on Wednesdays, so that the previous dose of rapamycin had taken place 48 hours prior to blood collection.ResultsFor all dogs, rapamycin Tmaxwas 2 hours. Median Cmaxwas 1.47 ng/ml (0.912 2.13), and the median AUC0-lastwas 15.7 ng*hr/mL (1.30 36.3). Due to sample size and timing, the only estimates related to elimination rate reported are for mean residence time with a median of 4.70 hrs (0.90 7.30).Conclusions and Clinical RelevanceA 0.025 mg/kg oral dose of rapamycin, administered three times a week, resulted in concentrations of rapamycin in the blood capable of being measured in ng/ml.

altmetric score

  • 1.5

author list (cited authors)

  • Evans, J. B., Morrison, A. J., Javors, M. A., Lopez-Cruzan, M., Promislow, D., Kaeberlein, M., & Creevy, K. E.

citation count

  • 1

complete list of authors

  • Evans, Jeremy B||Morrison, Ashley J||Javors, Martin A||Lopez-Cruzan, Marisa||Promislow, Daniel EL||Kaeberlein, Matt||Creevy, Kate E

Book Title

  • bioRxiv

publication date

  • January 2021